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Rapid Detection of BF Haplotypes by a Semi‐nested Polymerase Chain Reaction, which Causes Resistance/Susceptibility to Marek’s Disease in Chicken
Author(s) -
Jin Y.C.,
Wei P.,
Wei X.X.,
Zhao Z.Y.,
Li Y.
Publication year - 2010
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2010.02415.x
Subject(s) - haplotype , exon , microbiology and biotechnology , biology , nested polymerase chain reaction , polymerase chain reaction , marek's disease , virology , complementary dna , splice , genetics , gene , allele
A semi‐nested polymerase chain reaction (snPCR) assay was developed for the rapid detection of resistant/susceptible BF haplotypes to Marek’s disease (MD) using the cDNA samples from peripheral blood leucocytes, liver, spleen and heart from Xiayan homozygous chickens: A 11 , C 23 , D 8 and D 12 (resistant to MD), A 5 and B 21 (susceptible to MD). The snPCR was utilized to span alternative splicing‐out of the sequence encoding the second segment of the cytoplasmic part of the mature BF molecules (exon 7). This alternative exon 7 splice variant was detected in BF*A 5 and BF*B 21 (susceptible to MD), but not in the MD‐resistant BF*A 11 , BF*C 23 , BF*D 8 and BF*D 12 haplotypes, suggesting a potential role of exon 7 for the detection of resistant/susceptible BF haplotypes to MD.