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Functional Characterization of Murine CD25 Expressing B Cells
Author(s) -
Amu S.,
Gjertsson I.,
Brisslert M.
Publication year - 2010
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2010.02380.x
Subject(s) - il 2 receptor , cd19 , biology , b cell , immune system , population , antibody , immunology , b 1 cell , spleen , naive b cell , antigen , microbiology and biotechnology , t cell , antigen presenting cell , medicine , environmental health
B cells are an important part of both innate and adaptive immune system. Their ability to produce antibodies, cytokines and to present antigen makes them a crucial part in defence against pathogens. In this study, we have in naïve Naval Medical Research Institute mice functionally characterized a subpopulation of splenic B cells expressing CD25, which comprise about 1% of the total B cell compartment. Murine spleen cells were sorted into two highly purified B cell populations either CD19 + CD25 + or CD19 + CD25 − . We found that CD25 + B cells secreted higher levels of IL‐6, IL‐10 and INFγ in response to different TLR‐agonists, and were better at presenting alloantigen to CD4 + T cells. CD25 expressing B cells spontaneously secreted immunoglobulins of IgA, IgG and IgM subclass and had better migratory ability when compared with CD25 − B cells. In conclusion, our results demonstrate that CD25 + B cells are highly activated and functionally mature. Therefore, we suggest that this population plays a major role in the immune system and may belong to the memory B‐cell population.