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CD4 + and CD8 + Distribution Profile in Individuals Infected by Schistosoma mansoni
Author(s) -
OliveiraPrado R.,
Caldas I. R.,
TeixeiraCarvalho A.,
Andrade M. V.,
Gazzinelli A.,
CorreaOliveira R.,
CunhaMelo J. R.
Publication year - 2009
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2009.02247.x
Subject(s) - cd8 , peripheral blood mononuclear cell , biology , immunology , antigen , cd28 , microbiology and biotechnology , andrology , medicine , biochemistry , in vitro
Rationale: Patients with chronic Schistosoma mansoni infection show lower anti‐soluble egg antigen (SEA) proliferation responses and higher responses to soluble worm antigen preparation (SWAP). Objective: To compare the activation status and proliferation response of peripheral blood mononuclear cells (PBMC) of infected (XTO) and egg‐negative individuals (NI) living in the same endemic area. Methods: XTO ( n = 51) and NI individuals from the same geographical area ( n = 37) and healthy blood donors ( n = 22) were evaluated before and after stimulation with SEA and SWAP. The expression of activation markers (CD4 + HLADR + , CD8 high+ HLA‐DR + and CD8 + CD28 + ) and proliferation assay was assessed by flow cytometry. Findings : PBMC from infected patients showed lower frequency of CD4 + but no change in CD8 + T cells when compared with the healthy donor group. The ratio CD4 + /CD8 + was 1.3, 0.6 and 0.5 in healthy donors, infected and non‐infected individuals, respectively. The HLA‐DR + expression on CD8 + was higher in PBMC from infected and non‐infected individuals than from healthy donors, but similar in both total lymphocytes and CD4 + populations. No intergroup proliferation response differences were observed in CD4 + and CD8 + PBMC unstimulated and stimulated with SEA and SWAP. The SEA but not SWAP‐stimulated cells showed a decrease in the expression of phosphorylated extracellular signal‐regulated kinase (ERK1/2). Conclusions : XTO and NI individuals living in the same area presented a smaller per cent of CD4 + and a higher per cent of CD8 + cells. The activation by either CD8 high+ HLA‐DR + or CD8 high+ HLA‐DR + /CD8 + was enhanced and decreased in XTO and NI by CD8 + CD28 + and CD8 + CD28 + /CD8 + when compared with healthy donor. ERK phosphorylation was attenuated in XTO and NI individuals when stimulated with SEA but not SWAP.