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Modifying Antibody Specificity by Chain Shuffling of V H / V L between Antibodies with Related Specificities
Author(s) -
Christensen P. A.,
Danielczyk A.,
Ravn P.,
Larsen M.,
Stahn R.,
Karsten U.,
Goletz S.
Publication year - 2009
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2008.02164.x
Subject(s) - antibody , dna shuffling , immunoglobulin light chain , microbiology and biotechnology , biology , antigen , specific antibody , chemistry , biochemistry , computational biology , directed evolution , immunology , gene , mutant
Abstract Histo‐blood group antigens are important markers of developmental stages and as such also often of tumours. Generation of antibodies towards these carbohydrate structures is still a challenging task as they may lack specificity, affinity or are only of the IgM class. We have examined four own antibodies to Lewis Y/H type 2 for their fine specificities using a large panel of mono‐ and oligosaccharides. Sequence alignment to other antibodies with similar specificity revealed an overall limited variation, and that our antibodies constitute a novel set. Based on produced and analysed chimeric mouse–human antibodies, extensive chain shuffling experiments were performed in order to analyse influences of the respective H and L chains on the specificity of the antibodies, and to generate modified antibodies with improved properties. One chIgG1 out of the shuffled antibodies revealed improved specificity and markedly enhanced functional affinity to Lewis Y compared to the parental chIgG1 antibodies. Therefore, the combinatorial approach of chain shuffling provides a platform to improve specificity and/or affinity of anti‐carbohydrate antibodies.