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Liposome‐Encapsulated Antigens Induce a Protective CTL Response against Listeria monocytogenes Independent of CD4 + T Cell Help
Author(s) -
Grenningloh R.,
Darj A.,
Bauer H.,
Zur Lage S.,
Chakraborty T.,
Jacobs T.,
Weiss S.
Publication year - 2008
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2008.02112.x
Subject(s) - ctl* , listeria monocytogenes , mhc class i , cytotoxic t cell , antigen , liposome , antigen presentation , intracellular parasite , cross presentation , t cell , microbiology and biotechnology , intracellular , major histocompatibility complex , antigen processing , biology , immune system , virology , immunology , cd8 , biochemistry , bacteria , in vitro , genetics
Protection against intracellular pathogens is usually mediated by cytotoxic T lymphocytes (CTL). Induction of a protective CTL response for vaccination purposes has proven difficult because of the limited access of protein antigens or attenuated pathogens to the MHC class I presentation pathway. We show here that pH‐sensitive PE/CHEMS liposomes can be used as a vehicle to efficiently deliver intact proteins for presentation by MHC class I. Mice immunized with listerial proteins encapsulated in such liposomes launched a strong CTL response and were protected against a subsequent challenge with L. monocytogenes . Remarkably, the CTL response was induced independently of detectable CD4 + T cell help.