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The Unique Localization of ZFP185 at Uropod of Mouse T Lymphocytes
Author(s) -
Wang N.,
Zhang J.S.,
Zheng Q.,
Zhao Y.
Publication year - 2008
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2008.02071.x
Subject(s) - biology , microbiology and biotechnology
Zinc finger protein 185 (ZFP185), which contains a conservative Lin‐l 1, Isl‐1 and Mec‐3 (LIM) domain at its C‐terminus, is a member of mouse LIM protein family. Although brahma‐related gene 1(BRG1), the upstream regulation gene of ZFP185, is essential in the development and activation of T lymphocytes, there is no report on the involvement of ZFP185 in lymphocyte activation. The expression of ZFP185 protein was up‐regulated significantly in T lymphocytes during their activation by phorbol 12‐myristate 13‐acetate (PMA)/ionomycin, compared with non‐activated T lymphocytes, as determined by western blot assays. However, over‐expression of ZFP185 in EL4 cells showed no significant effect on the cell proliferation. Importantly, expression of ZFP185‐enhanced green fluorescent protein fusion protein in EL4 cells showed that ZFP185 uniquely localized in the uropod of lymphocytes as observed with a two‐photon laser scanning confocal microscope. The direct interaction between ZFP185 and actin was supported by co‐immunoprecipitation and immunofluorescence assays. The special distribution of ZFP185 in uropod indicates the potential involvement of ZFP185 in T‐cell morphology and movement.