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Adjuvant Effects of Bacillus Calmette–Guerin DNA or CpG‐oligonucleotide in the Immune Response to Taenia solium Cysticercosis Vaccine in Porcine
Author(s) -
Guo Y.J.,
Wu D.,
Wang K.Y.,
Sun S.H.
Publication year - 2007
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2007.02013.x
Subject(s) - taenia solium , adjuvant , immunogenicity , dna vaccination , cpg site , virology , immune system , biology , immunization , antigen , immunology , cysticercosis , dna methylation , gene , zoology , biochemistry , gene expression
The immune stimulation properties of CpG‐oligonucleotides (CpG‐ODN) containing a central unmethylated CpG motif could be useful for vaccination against parasite infection. However, the high cost of synthetic CpG‐ODN has limited its use in veterinary vaccines. In this study, we investigated whether genomic DNA derived from Mycobacterium bovis bacillus Calmette–Guerin (BCG‐DNA) could be used as an effective adjuvant to enhance the immunogenicity and the protective capacity of recombinant cC1 antigen (rcC1) against pig cysticercosis. Pigs were vaccinated with rcC1 plus CpG‐containing DNA adjuvants (BCG‐DNA or CpG‐ODN) or rcC1 alone. Immunization with rcC1 alone induced a Th1‐biased response, whereas coadministration of rcC1 with BCG‐DNA or CpG‐ODN increased levels of IgG2, IFN‐γ, percentage of CD8 + and specific proliferation of peripheral blood mononuclear cells. Four weeks after the last immunization, pigs were infected with Taenia solium eggs. A high level of protection (81%) was induced by rcC1 immunization that was not significantly increased by the CpG‐containing DNA. These data indicate that coadministration of rcC1 plus BCG‐DNA or CpG‐ODN significantly enhanced Th1 response but did not improve the level of the protection induced.