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The Human Immunomodulatory CD25 + B Cell Population belongs to the Memory B Cell Pool
Author(s) -
Amu S.,
Tarkowski A.,
Dörner T.,
Bokarewa M.,
Brisslert M.
Publication year - 2007
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2007.01946.x
Subject(s) - il 2 receptor , cd86 , cd80 , immunology , cd20 , population , b cell , cd19 , naive b cell , cytokine , cd38 , biology , microbiology and biotechnology , medicine , t cell , cd40 , cytotoxic t cell , antigen presenting cell , antibody , immune system , in vitro , stem cell , biochemistry , environmental health , cd34
We have shown that human CD20 + 25 + B cells display immunomodulatory properties. The aim of this study was to investigate if CD25 + B cells are found within the CD27 memory B cell population, and to analyse pattern of their cytokine production. B cells isolated from healthy subjects, rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients were analysed regarding the frequency of CD25 + B cells within certain B cell subsets. Purified CD25 + B cells from healthy subject were used in vitro to evaluate their production of immunomodulatory cytokines. In healthy subjects the majority (60%) of memory B cells (CD20 + 27 + ) also co‐expressed CD25 while only 10–20% of the naïve B cells (CD20 + 27 − ) and plasmablasts (CD20–27 + ) expressed CD25. In RA and SLE patients, we found that 51% and 48%, respectively, co‐expressed CD25 in the memory population, whereas only 11% and 9% co‐expressed CD25 in the naïve B cell population. Phenotypic analysis of the CD20 + 25 + 27 + and CD20 + 25 + 27 − cells using CD10, CD24, CD38, CD45, CD71, CD80, CD86, CD95, CD138, BAFF‐R, TACI, IgA, IgD, IgG and IgM showed that CD20 + 25 + 27 + B cells preferentially represent highly activated, Ig class switched memory B cells. Cytokine profile analysis showed that CD25 + B cells secreted significantly higher levels of IL‐10 versus CD25 − B cells. In contrast, TGF‐ β 1 secretion was similar between the CD25 + and CD25 − sub‐populations. In conclusion, CD20 + 25 + B cells constitute a unique subpopulation preferentially occurring among CD20 + 27 + memory B cells. We suggest that CD25 can be used as a marker for a memory B cell subset.