Premium
Modulatory Effect of Killed Propionibacterium acnes and Its Purified Soluble Polysaccharide on Peritoneal Exudate Cells from C57Bl/6 Mice: Major NKT Cell Recruitment and Increased Cytotoxicity
Author(s) -
Ananias R. Z.,
Rodrigues E. G.,
Braga E. G.,
Squaiella C. C.,
Mussalem J. S.,
Longhini A. L. F.,
Travassos L. R.,
LongoMaugéri I. M.
Publication year - 2007
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2007.01939.x
Subject(s) - propionibacterium acnes , cytotoxicity , cytotoxic t cell , microbiology and biotechnology , in vitro , cd8 , natural killer t cell , biology , adjuvant , immune system , immunology , chemistry , bacteria , biochemistry , genetics
Propionibacterium acnes has been described as a potent adjuvant to immune responses in vitro and in vivo . Presently, we analysed the modulation of peritoneal exudate cells (PEC) by heat‐killed P. acnes or its purified soluble polysaccharide (PS), both injected intraperitoneally in C57Bl/6 mice, aiming at their recruitment and cytotoxicity. Both treatments induced an increase in macrophages, immature dendritic cells, B1a lymphocytes and NK1.1 + CD3 + cells. The bacterium caused a remarkable increase in a NK1.1 + CD3 + CD4 − CD8 − cells subpopulation, whereas the PS component seemed responsible for the recruitment of mainly macrophage cells. To assess P. acnes and PS adjuvant effect on PEC cytotoxicity we evaluated their in vitro effect on murine B16F10 melanoma cells. The effector cells from the heat‐killed bacteria and PS‐treated groups lysed melanoma cells in co‐cultures with PEC. Mice genetically deficient in IFN‐ γ , when stimulated with P. acnes or PS, had reduced PEC cytotoxicity, and the cytotoxic effect was completely abrogated in PEC from iNOS −/− mice. The tumoricidal activity of PEC from P. acnes ‐treated mice was mediated by macrophages and NKT cells stimulated with IL‐12. In PS‐treated mice the cytotoxicity was mediated mainly by macrophages. Moreover, both treatments increased IL‐4 and IFN‐ γ production by NKT cells. In conclusion, we show that P. acnes act mainly by recruiting and activating NKT double‐negative cells in PEC, which were shown to be tumoricidal in vitro when induced by IL‐12. Macrophages induced by both P. acnes and PS have their antitumour effect dependent on NO production.