Gene Gun‐delivered  p GM‐CSF Adjuvant Induces Enhanced Emigration of two Dendritic Cell Subsets from the Skin | Zendy

Matthews K. | Zendy; Bailey S. L. | Zendy; Gossner A. G. | Zendy; Watkins C. | Zendy; Dalziel R. G. | Zendy; Hopkins J. | Zendy

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Gene Gun‐delivered p GM‐CSF Adjuvant Induces Enhanced Emigration of two Dendritic Cell Subsets from the Skin

Author(s) -

Matthews K.,

Bailey S. L.,

Gossner A. G.,

Watkins C.,

Dalziel R. G.,

Hopkins J.

Publication year - 2007

Publication title -

scandinavian journal of immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.934

H-Index - 88

eISSN - 1365-3083

pISSN - 0300-9475

DOI - 10.1111/j.1365-3083.2006.01892.x

Subject(s) - cd11c , cytokine , dendritic cell , lymph , immunology , gene gun , medicine , cd86 , biology , pathology , phenotype , immune system , t cell , gene , immunization , dna vaccination , biochemistry

Two subsets of sheep afferent lymph dendritic cells (DC) are defined by the differential expression of CD172a and CD45RA. The majority (∼70%) of CD172a + subset is CD45RA/CD11c + /CD207 + /TLR4 + . The CD172a − DC are CD45RA + /CD207 − and express low levels of CD11c and CD86. Real‐time RT‐PCR showed that CD172 + DC produce IL‐1 β and IL‐10 and high levels of IL‐18 but almost no IL‐12 p40 ; CD172a − DC express IL‐12 p40 but no IL‐10 and low levels of IL‐1 β and IL‐18. Gene gun‐delivered granulocyte‐macrophage colony‐stimulating factor ( p GM‐CSF) caused an early rise in the output of CD172a + DC, changes to DC phenotype and significant increases in the levels of expression cytokine transcripts. However, p GM‐CSF did not affect any qualitative changes to cytokine expression, CD172a + DC remained IL‐10 + /IL‐12 p40 − and the CD172 − DC remained IL‐10 − /IL‐12 p40 + .

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