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Cytokine Profile of T lymphocytes from Peripheral Blood and Bronchoalveolar Lavage Fluid in Patients with Active Pulmonary Tuberculosis
Author(s) -
Boras Z.,
Juretić A.,
Gagro A.,
Pavelić Lj
Publication year - 2007
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2006.01890.x
Subject(s) - medicine , tuberculin , immunology , bronchoalveolar lavage , cd8 , cytokine , peripheral blood mononuclear cell , tuberculosis , peripheral blood lymphocyte , lymphocyte , interferon gamma , immune system , lung , pathology , biology , in vitro , biochemistry
The possible immunological relationship between the pattern of Th1/Th2 cytokine production and tuberculin reactivity was assessed in patients with active Mycobacterium tuberculosis infection. The production of the intracellular cytokines interferon (IFN)‐ γ and interleukin‐4 (IL‐4) was measured in CD4 + and CD8 + T cells obtained from peripheral blood and bronchoalveolar lavage fluid (BALF) of 20 tuberculin skin‐positive patients and compared with the findings recorded in nine tuberculin skin‐negative patients with active pulmonary tuberculosis. Upon stimulation with phorbol 12‐myristate acetate/ionomycin for 6 h, tuberculin‐negative patients had a significantly higher proportion of IFN‐ γ ‐producing CD4 + T lymphocytes in BALF than in peripheral blood, while both CD4 + and CD8 + T‐lymphocyte subsets in BALF of tuberculin‐positive patients secreted more IFN‐ γ than their peripheral blood counterparts. Tuberculin‐negative patients had a significantly higher proportion of IFN‐ γ ‐producing CD4 + T lymphocytes in peripheral blood than tuberculin‐positive patients. There was no significant difference in the production of IFN‐ γ by BALF CD4 + T lymphocytes, or by either peripheral blood or BALF CD8 + T lymphocytes. In two tuberculin‐negative patients, peripheral blood CD4 + T lymphocytes produced IL‐4. Study results suggested a higher immune activity in the blood of tuberculin‐negative patients, with an increased lymphocyte activity in BALF versus peripheral blood in both patient groups.