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Long‐term Haematopoietic Reconstitution and Survival without Interleukin‐7 in a Murine Syngeneic Bone Marrow Transplantation Model
Author(s) -
Feyen O.,
Göbel U.,
Schneider D. T.,
Burdach S. E. G.,
Niehues T.
Publication year - 2006
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2006.01847.x
Subject(s) - haematopoiesis , bone marrow , cd8 , transplantation , immunology , biology , interleukin 2 , cancer research , medicine , immune system , stem cell , microbiology and biotechnology
We created a syngeneic mouse bone marrow transplantation (BMT) model to examine the effect of endogenous interleukin‐7 (IL‐7) on long‐term (≥140 days) haematopoietic reconstitution and survival after BMT. Wild‐type (WT) IL‐7 +/+ and knockout (KO) IL‐7 −/− mice were lethally irradiated and transplanted with bone marrow. Survival is best (85.7%) in the group WT grafts transplanted into WT recipients (WT→WT) with a trend towards poorer survival in the other groups (WT→KO: 60%, KO→WT: 50%, KO→KO: 45.5%, differences statistically not significant). If the recipient is deficient for IL‐7‐producing cells, T‐ and B‐cell reconstitution remain incomplete. If the graft lacks IL‐7‐producing cells there is a significant delay in T‐ and NK‐cell reconstitution. Interestingly, in the absence of IL‐7, T‐cell reconstitution is neither delayed nor incomplete because of an expansion of TCR α β + /CD4 − /CD8 − double negative T cells. Long‐term survival and lymphocyte reconstitution after syngeneic BMT can occur despite the absence of IL‐7.