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Mycoplasma mycoides ssp. mycoides Biotype Small Colony‐Secreted Components Induce Apoptotic Cell Death in Bovine Leucocytes
Author(s) -
Dedieu L.,
Chapey E.,
BalcerRodrigues V.
Publication year - 2005
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2005.01690.x
Subject(s) - mycoplasma mycoides , biology , contagious bovine pleuropneumonia , apoptosis , microbiology and biotechnology , programmed cell death , mycoplasma , cytotoxicity , immunology , biochemistry , in vitro
Contagious bovine pleuropneumonia, caused by Mycoplasma mycoides ssp. mycoides biotype small colony ( Mmm SC), is one of the most serious cattle diseases in Africa. Several observations suggested that Mmm SC had evolved an efficient way to escape the bovine immune responses by triggering host‐cell cytotoxicity. This study was implemented to determine whether the cytotoxic effect was due to apoptotic cell death. To that end, bovine blood cells were cultured for up to 3 days in the presence of viable or heat‐killed Mmm SC compared to unstimulated cultures. The findings provided evidence for a viable Mmm SC‐induced, time‐dependent apoptosis in bovine blood leucocytes, whereas heat‐killed Mmm SC had no effect. Morphological and physiological changes (evidenced by TUNEL and annexin V staining) typical of apoptosis were observed in response to viable Mmm SC. All the lymphocyte subsets as well as the monocyte/granulocyte subset exhibited extensive apoptosis after exposure to viable Mmm SC. Our results demonstrated a potential role for Mmm SC‐secreted components as pathogenic factors able to induce programmed cell death in bovine blood leucocytes.