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DNA Vaccination Using Bacillus Calmette‐Guerin‐DNA as an Adjuvant to Enhance Immune Response to Three Kinds of Swine Diseases
Author(s) -
Zhang S.,
Guo Y.J.,
Sun S.H.,
Wang K.Y.,
Wang K.H.,
Zhang Y.,
Zhu W.J,
Chen Z.H.,
Jiang L.
Publication year - 2005
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2005.01674.x
Subject(s) - dna vaccination , immune system , adjuvant , immunology , antibody , biology , vaccination , virology , immunity , antigen , humoral immunity , immunization
In order to enhance the immune efficacy of DNA vaccination, experiments were conducted to investigate the regulating effects of Bacillus Calmette‐Guerin (BCG)‐DNA as an adjuvant on immune responses of mice against foot‐and‐mouth disease (FMD), Aujeszky's disease (AjD) and classical swine fever (CSF). BCG‐DNA was purified from BCG by ion‐exchange chromatography. Three DNA vaccines (pVSG, pVgD and pVE2) against the respective infection were constructed, and BCG‐DNA was coimmunized to mice by muscle injection. The results showed that titres of specific immunoglobulin (Ig)G to the vaccines mounted remarkably in the sera of the adjuvant covaccinated mice ( P < 0.01). Antibody isotype IgG2a and IgG1 also increased, respectively, in mice coimmunized with BCG‐DNA compared with those of the control groups ( P < 0.01). Cellular immune cytokine interferon‐γ and cytotoxic T lymphocytes were detected in coimmunized BCG‐DNA groups ( P < 0.05). Whereas interleukin‐4, humoral immune cytokine, was not significant ( P > 0.05). These results suggest that codelivery of BCG‐DNA with DNA vaccines against FMD, AjD and CSF can enhance the induction of antigen‐specific, especially, cell‐mediated immunity.