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Lipopolysaccharide Effectively Up‐Regulates B7‐1 (CD80) Expression and Costimulatory Function of Human Monocytes
Author(s) -
SCHMITTEL A.,
SCHEIBENBOGEN C.,
KEILHOLZ U.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03714.x
Subject(s) - lipopolysaccharide , cd80 , tumor necrosis factor alpha , cd86 , immunology , biology , monocyte , interferon gamma , microbiology and biotechnology , cytokine , immune system , t cell , cytotoxic t cell , cd40 , in vitro , biochemistry
The influence of lipopolysaccharide (LPS) and various cytokines on the expression of the costimulatory molecule B7‐1 and intercellular adhesion molecule‐1 (ICAM‐1), lymphocyte function associated antigen‐3 (LFA‐3) and human histocompatibility leucocyte antigen‐DR (HLA‐DR) on human monocytes and their effect on the costimulatory function was investigated. Freshly isolated human monocytes constitutively express ICAM‐1, LFA‐3 and HLA‐DR, but no B7‐1. B7‐1 expression was up‐regulated by LPS and, to a lesser extent, by interferon‐γ (IFN‐γ). The other stimuli tested, including IFN‐α, granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), tumour necrosis factor‐α (TNF‐α) and GM‐CSF + TNF‐α, did not influence expression of B7‐1 on monocytes. ICAM‐1 and HLA‐DR were up‐regulated by IFN‐γ and LPS; LFA‐3 expression was not influenced. LPS also effectively enhanced costimulatory function of monocytes as determined in the tetanustoxoid (TT) assay. Blocking of B7 by CTLA‐4Ig inhibited the LPS‐induced enhancement of costimulatory function almost completely. Our results indicate that the LPS‐mediated up‐regulation of the costimulatory function of human monocytes is mediated by B7. This mechanism may be important for host defence against Gram‐negative bacteria.