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Specific Interferon‐Gamma Producing CD4 + and CD8 + T Cells After Autologous EBV‐B Stimulation: The Necessity of Restimulation
Author(s) -
VOOIJS W. C.,
NOOTEBOS M. G.,
WERNERT H.,
GAST G. C.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03701.x
Subject(s) - cytotoxic t cell , interferon , stimulation , cd8 , interferon gamma , microbiology and biotechnology , biology , immunology , virology , antigen , chemistry , cytokine , in vitro , endocrinology , biochemistry
The response of T cells to produce interferon‐γ, to proliferate and to become cytotoxic after specific stimulation with low dose (2%) autologous EBV‐B cells was investigated in 15 EBV seropositive and five seronegative patients. A significantly higher number of interferon‐γ producing cells (56 ± 24 per 10 5 T cells) were found in a spot ELISA in EBV positive than in EBV negative patients (7 ± 2 spots, P<0.01) and it was only found with restimulation after 5–12 days of primary culture. No correlation was found between the extent of interferon‐γ production, cytotoxicity or profileration. Specificity of EBV‐induced interferon‐γ production was demonstrated by comparison of the response to allogeneic EBV‐B cells or IL‐2 in the reslimulation phase. The response was stronger in CDS + T cells than in CD4 + T cells and could be blocked in the restimulation phase with HLA class I and class II antiserum, respectively.