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Secretion of MPB64 Antigen by a Recominant Clone of Mycobacterium Smegmatis : Characterization and Application for the Diagnosis of Tuberculosis
Author(s) -
TASAKA H.,
SHIGETO E.,
MATSUO K.,
YAMAGUCHI R.,
HAGA S.,
YAMAZAKI A.,
YAMAZAKI T.,
NAGAI S.,
NAKAMURA R. M.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03684.x
Subject(s) - mycobacterium smegmatis , microbiology and biotechnology , mycobacterium tuberculosis , antigen , mycobacterium , biology , bacteria , tuberculosis , virology , medicine , immunology , pathology , genetics
MPB64, a specific antigen to Mycobaeterium tuberculosis complex (TB complex), was produced and secreted by a clone of M. smegmatis ‐MPB64 where the structural gene of MPB64 was inserted using a new mycobacteria, E. coli shuttle plasmid pNIS vector. Antibodies against the recombinant MPB64 (rMPB64) were used for the reverse particle latex agglutination (RPLA) test to detect the MPB64 antigen rapidly. RPLA tests were applied to the stock cultures and the clinical isolates of mycobacteria to identify TB complex. RPLA with anti‐MPB64 antibody‐coated latex beads completely distinguished TB complex from other mycobacteria. Thus, it is suggested that RPLA with anti‐MPB64 antibody would be a new, easy and inexpensive method for the rapid diagnosis of tuberculosis.