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V‐Region and Class Specific RT‐PCR Amplification of Human Immunoglobulin Heavy and Light Chain Genes from B‐Cell Lines
Author(s) -
WATKINS B. A.,
DAVIS A. E.,
FIORENTINI S.,
REITZ M. S.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03678.x
Subject(s) - immunoglobulin light chain , gene , biology , microbiology and biotechnology , antibody , immunoglobulin class switching , polymerase chain reaction , immunoglobulin heavy chain , heavy chain , b cell , immunoglobulin gene , somatic cell , genetics
We have designed and tested primers that amplify complete human kappa and lambda light chain genes, and human Fd fragments from gamma, mu and alpha heavy chain genes. These primers were tested for efficiency and specificity on monoclonal sources of human immunoglobulin RNA, obtained from human B‐cell lines of known immunoglobulin gene expression. Analysis of the sequences derived from these B‐cells confirms the specificity of the PCR primers and the extent of somatic mutation seen in different B‐cell malignancies supports existing concepts for differing aetiologies in the tumours concerned.