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Stimulation of Human Peripheral Lymphocytes via CD3 and Soluble Antigen Abrogates Specific Antibody Production by Reducing Memory B Cell Numbers
Author(s) -
INGVARSSON S.,
LAGERKVIST A. C. SIMONSSON,
CARLSSON R.,
BORREBAECK C. A. K.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03664.x
Subject(s) - stimulation , antigen , antibody , immunology , peripheral , cd3 , cell , peripheral blood , chemistry , biology , microbiology and biotechnology , medicine , neuroscience , cd8 , biochemistry
Human B cells are polyclonally activated in vitro by T cells stimulated with immobilized anti‐CD3 monoclonal antibodies. We have analysed the effect of CD3 ligation on the production of antigen‐specific antibodies, using peripheral blood lymphocytes from tetanus toxoid vaccinated blood donors. High levels of antigen‐specific antibodies were obtained after stimulation with anti‐CD3 antibodies for 7 days. Addition of a soluble recall antigen did not affect the total amount of Ig produced, but dramatically decreased the antigen‐specific response. The addition of IL‐2, IL‐4, anti‐CD40 or anti‐CD28 antibodies or the removal of antigen did not restore the B cell response. Analysis using limiting dilution of B cells showed that the frequency of antigen‐specific memory B cells decreased significantly in cultures stimulated with antigen. The antigen‐specific B cell response could be completely restored only if the soluble antigen was cross‐linked on the surface of the B cells. These results suggested that peripheral memory B cells were eliminated or anergized in the presence of soluble antigen.