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Characterization of Chicken CD8‐Specific Monoclonal Antibodies Recognizing Novel Epitopes
Author(s) -
LUHTALA M.,
KOSKINEN R.,
TOIVANEN P.,
VAINIO O.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03641.x
Subject(s) - epitope , monoclonal antibody , cd8 , microbiology and biotechnology , biology , major histocompatibility complex , antigen , thymocyte , antibody , t cell , cytotoxic t cell , immunology , biochemistry , immune system , in vitro
CDS is a heterodimeric cell surface glycoprotein expressed primarily on thymocytes and a subpopulation of mature T lymphocytes. It binds to the invariant part of the major histocompatibility complex class I molecule and participates in antigen recognition by the major histocompatibility complex class I restricted T cells. As in mammalian species, the majority of chicken thymocytes express both CD4 and CD8, whereas peripheral T cells are either CD4‐ or CD8‐positive. We have created a panel of mouse monoclonal antibodies detecting different cell surface epitopes on chicken CD8. The antibodies precipitate a 32–34 kDa dimeric protein from surface labelled thymocytes under reducing conditions. The identical N‐deglycosylation pattern confirms that these MoAb precipitate the same heterodimeric molecule from chicken thymocyte lysates. Binding of 11–38 and 11–39 MoAb to peripheral blood T cells is totally inhibited by 11–39 and previously characterized CT8 and EP72 MoAb, further confirming their CD8 specificity. CD8α‐chain specificity of MoAb 11–39, 11–38, 11–30 and 11–13 is conclusively proven by staining COS‐cells transfected with a plasmid containing CD8α cDNA. However, MoAb 11–13, 11–30 and 11–38 do not compete with MoAb 11–39 in binding to CD8. These results demonstrate recognition of different epitopes by these MoAb. Monoclonal antibodies detecting novel epitopes on chicken CD8 provide a valuable tool for further studies on T cell development.