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Gliadin‐Specific T Cell Responses in Peripheral Blood of Healthy Individuals Involve T Cells Restricted by the Coeliac Disease Associated DQ2 Heterodimer
Author(s) -
JENSEN K.,
SOLLID L. M.,
SCOTT H.,
PAULSEN G.,
KETT K.,
THORSBY E.,
LUNDIN K. E. A.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03640.x
Subject(s) - gliadin , coeliac disease , immunology , t cell , immune system , peripheral blood , peripheral blood mononuclear cell , peripheral , in vitro , biology , medicine , disease , gluten , biochemistry
Coeliac disease (CD) is probably caused by an abnormal immune response towards wheat gliadin in the small intestine. We found that gliadin‐specific T cells from the small intestinal mucosa of HLA‐DQ2 positive CD patients were almost exclusively restricted by the disease‐associated DQ2 molecule. In the peripheral blood of CD patients, a large proportion of gliadin‐specific T cells were found to be restricted by DQ molecules, including DQ2, but many were instead restricted by DR or DP molecules of the patient. We have now investigated gliadin‐specific T cell responses in peripheral blood from healthy individuals. Four of 20 persons tested had strong in vitro responses and were used as donors for gliadin‐specific T cell clones. We found gliadin‐specific T cells restricted by the CD‐associated DQ2 molecule in peripheral blood for two of these four individuals. It is the presence of such T cells also in the small intestinal mucosa which seems typical of CD.