Premium
A Correlation Between the Expression of the bcr‐abl Chimeric Gene and Severity of the Clinical State of CML Patients with Time
Author(s) -
AMIKAM D.,
HENIG C.,
CARTER A.,
SHARON R.,
BENISHAI Z.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03603.x
Subject(s) - breakpoint cluster region , polymerase chain reaction , reverse transcription polymerase chain reaction , southern blot , reverse transcriptase , real time polymerase chain reaction , abl , oncogene , cancer research , western blot , biology , microbiology and biotechnology , gene , medicine , gene expression , genetics , receptor , cell cycle , tyrosine kinase
Using the reverse polymerase chain reaction, four Ph positive CML patients were followed for 2 years; a correlation between the severity of the clinical state and the b3a2 expression was noted with time. Additionally, amplification of the c‐myc proto‐oncogene was observed, using Southern blot analysis, in one patient prior to his entry to the blast phase. No reorganization of the bcr‐abl rearrangement site was found in the latter patient. The data suggest that a routine follow‐up of CML patients using the Southern blot analysis and the reverse transcription‐polymerase chain reaction might be of importance in evaluating the progression of the disease.