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Anti‐GD 3 Antibodies are Potent Activators of Human γ/δ and α/β Positive T Cells
Author(s) -
SCHLAAK J. F.,
CLAUS C.,
MEYER K.H.,
BÜSCHENFELDE ZUM,
DIPPOLD W.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03595.x
Subject(s) - cd28 , t cell , cd8 , cytokine , cytotoxic t cell , t cell receptor , microbiology and biotechnology , biology , antibody , cd3 , interleukin 21 , antigen , antigen presenting cell , chemistry , immunology , immune system , biochemistry , in vitro
The ganglioside GD3 has a variety of biological functions. These include stimulatory effects is on proliferation, natural killer activity and cytokine production by freshly isolated peripheral T cells. In this study we have characterized anti‐GD3 antibody (MoAb Z21) mediated effects on T cell clones. Our data indicate that α/β TCR CD4 + and CD8 + as well as γ/δ TCR positive T cells can be stimulated resulting in proliferation and cytokine production. This effect could be blocked by cyclosporin A and did not involve the LFA‐3 or CD4 molecule. Apart from IFN‐γ and IL‐2 production by T helper I and T helper 0 cells we have observed production of IL‐4 and IL‐10 by T helper 2 cells indicating that the GD3 molecule is not a marker for a certain functional T cell subset. In contrast to anti‐CD3 mediated activation, the responsiveness of T cells to stimulation via GD3 was dependent on the cell surface expression of the molecule and could be enhanced by costimulation via CD2, CD3, CD26 or CD28. In addition, anti‐GD3 antibodies delivered a potent costimulatory signal for antigen‐induced proliferation of CD4 + T lymphocytes. In summary, our experiments illuminate the mechanisms of anti‐GD3 antibody induced T cell activation.