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Acute Graft‐versus‐Host Reaction in SCID Mice Leads to an Abnormal Expansion of CD8 + Vβ14 + and a Broad Inactivation of Donor T Cells Followed by a Host‐Restricted Tolerance and a Normalization of the TCR Vβ Repertoire in the Chronic Phase
Author(s) -
SCHNEIDER M. K. J.,
GRÖNVIK K.O.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03581.x
Subject(s) - cd8 , spleen , population , immunology , cytotoxic t cell , microbiology and biotechnology , transplantation , graft versus host disease , lymph , antigen , t lymphocyte , t cell , biology , chemistry , medicine , immune system , pathology , in vitro , biochemistry , environmental health
The persistence and selection of allogeneic CBA/J T lymphocytes were studied during graft‐versus‐host (GvH) reaction in immunodeficient C. B‐17 SCID (SCID) mice. After neonatal injection the donor cells primarily migrated to the spleen plus lymph nodes (SL) and the thymus of the recipients. Thirteen days post engraftment, CD8 + cells in SL had increased five times in cell number with an 18‐fold increase of CD8 + Vβ14 + cells, paralleled by clinical signs of GvH disease (GvHD). Donor lymphocytes from these mice were proliferative unresponsive to allogeneic Balb/c or C57B1/6 SL cells, whereas 8 weeks post injection the tolerance was confined to H‐2 d specific donor cells. Here, spleens had a total cell content similar to untreated SCID mice but the average percentage of donor cells had reached 25%. Moreover, the CD4/CD8 cell ratio in the donor population in SL and thymus had changed to normal and the TCR Vβ repertoire was similar to that of the originally injected cells. Following secondary transfer into syngeneic CBA/Ca nu/nu recipients donor cells regained a significant but reduced response to H‐2 d stimulators indicating that the antigen specific tolerance of allogeneic donor cells in the SCID mice was due, at least in part, to a reversible state of anergy.