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Cytokine Gene Expression During Infeetion of Miee Lacking CD4 and/or CD8 with Trypanosoma cruzi
Author(s) -
ROTTENBERG M. E.,
SPORRONG L.,
PERSSON I.,
WIGZELL H.,
ÖRN A.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03549.x
Subject(s) - trypanosoma cruzi , cd8 , biology , messenger rna , cytotoxic t cell , microbiology and biotechnology , cytokine , lymphokine , gene expression , nitric oxide , interferon gamma , gene , immunology , immune system , endocrinology , biochemistry , parasite hosting , in vitro , world wide web , computer science
The expression of lymphokine genes during infection of virulent (Tulahuén) or mild ( CA‐I ) strains of T. cruzi was studied in mice lacking CD4 and/or CD8 molecules. The increased susceptibility of CD4 − and CD4 − CD8 − mice to infection with CA‐I or Tuiahuen was parallelled by diminished IFN‐γ mRNA levels. Nitric oxide release and inducible nitric oxide synthase mRNA accumulation by cells from Tulahuen infected CD4 − mice was also diminished. CD8 − (but not CD4 − CD8 − mice) showed an increased IL‐4 and IL‐10 mRNA accumulation upon infection with both strains of T. cruzi. A T h 2‐like’ response (higher IL‐4 and IL‐10 mRNA to IFN‐γ mRNA ratio), was also observed when cells from non‐infected CD8 ‐ mice were stimulated with T cell mitogens.