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Cell‐Cycle Kinetics of Proliferating Mouse B Lymphocytes In Vitro
Author(s) -
KÄLLBERG E.,
LEANDERSON T.
Publication year - 1995
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1995.tb03539.x
Subject(s) - in vitro , microbiology and biotechnology , cell cycle , population , biology , kinetics , sepharose , cell , chemistry , biochemistry , enzyme , medicine , physics , environmental health , quantum mechanics
Resting mouse B lymphocytes were stimulated in vitro with Upopoiysaccharide, Sepharose‐coupled anti‐γ antibodies or a combination of the two. B lymphocytes stimulated with anti‐γ entered the cell‐cycle with more rapid kinetics and at a higher frequency than did the corresponding cell population stimulated with lipopolysaccharide. Using cell cycle analysis after DNA staining combined with an M phase block, the cell‐cycle kinetics of in vitro cultured B‐lymphocytes was studied. The labelling index of lipopoly saccharide stimulated B lymphocytes was 60% while that for anti‐γ Sepharose stimulated cells was 85%. The generation time of the actively cycling population from both types of cultures was constant and was of the order of 18 h. Thus, the fraction of B lymphocytes induced to proliferate in vitro varies depending on the stimulus, while the growth kinetics of the actively proliferating populations are remarkably constant.