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Human Th0‐type T Helper‐Cell Clone Supports Antigen‐Specific Immunoglobulin Production in Scid/beige‐hu Mice
Author(s) -
CHIN L.T.,
IFVERSEN P.,
KRISTENSSON K.,
WAHREN B.,
CARLSSON R.,
BORREBAECK C. A. K.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03500.x
Subject(s) - biology , clone (java method) , toxoid , antigen , t cell , lymphokine , t helper cell , virology , cytotoxic t cell , microbiology and biotechnology , t lymphocyte , cd8 , interleukin 21 , immunology , immune system , in vitro , immunization , dna , biochemistry , genetics
Tetanus toxoid‐specific T cells have been generated from human splenic lymphocytes by an initial 6‐day stimulation period with antigen, followed by a proliferation period with recombinant IL‐2 and human feeder cells. Proliferating T cells were subsequently cloned by limiting dilution. A human T‐cell clone that was functionally characterized showed: (i) a specific proliferative response to tetanus toxoid in the presence of autotogous Epstein—Barr virus (EBV)‐transformed lymphoblastoid cells; (ii) a phenotype characteristic for the helper/inducer CD4 + /CD8 − /CD45RO + T cells, and (iii) a lymphokine profile, as determined by mRNA analysis, representative of Th0‐like human CD4 + T helper cells. This tetanus toxoid‐specific T‐cell clone which showed antigen‐dependent helper activity for antibody production by autologous B cells in vitro could also provide T‐cell help to antigen‐specific human B cells transplanted into severe combined immunodeficiency (scid/beige) mice.