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Analysis of the Capacity to Produce IL‐3 in Murine AIDS
Author(s) -
NEUENSCHWANDER A. U.,
MARKER O.,
THOMSEN A. R.,
Thomsen Allan Randrup
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03482.x
Subject(s) - stimulation , splenocyte , biology , immunology , virus , virology , microbiology and biotechnology , endocrinology , immune system
Adult C57BL/6 mice infected with LP‐BM5 murine leukaemia virus represent a model of murine AIDS (MAIDS). In this study we have analysed the capacity of CD4 + T cells from infected mice to produce IL‐3 following stimulation with ConA for 24–72 h. In contrast to the position with IL‐2, the production of which is markedly impaired during LP‐BM5 infection, similar levels of IL‐3 were measured in culture supernatants of splenocytes from infected and uninfected mice harvested at 24 h of stimulation. Forty eight and 72 h of ConA stimulation led to increasing levels of IL‐3 being measured in cultures from uninfected mice, whilst in cultures from infected animals, IL‐3 levels remained stagnant. Similar results were obtained 4, 8 and 13 weeks post‐infection. In view of the fact that parallel experiments revealed markedly impaired proliferative responses to ConA during MAIDS, we conclude that IL‐3 production is basically intact at the cellular level in T cells during MAIDS; but when in a situation requiring clonal expansion of the activated T cells, IL‐3 production will be inhibited owing to the impaired capacity for proliferation.