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Evolutionarily Conserved Function of CD28 in αβ T Cell Activation
Author(s) -
ARSTILA T. P.,
VAINIO O.,
LASSILA O.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03476.x
Subject(s) - cd28 , concanavalin a , t cell receptor , cd3 , biology , t cell , monoclonal antibody , microbiology and biotechnology , stimulation , immunology , antibody , antigen , cd8 , immune system , endocrinology , biochemistry , in vitro
The functional role of the chicken homologue of CD28 was studied. It is expressed on all thymocytes, and both Vβ1‐ and Vβ2‐family expressing peripheral αβ T cells. Peripheral γβ T cells are CD28‐negative. Monoclonal antibody against CD28 had a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MoAb against TCR. Vβl and Vβ2 expressing cells responded equally well to stimulation with anti‐CD28 in combination with PMA. These responses were resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3. These data indicate a striking conservation of the costimulatory function of CD28 and emphasize the importance of this costimulatory pathway.