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T Cell Receptor Vα Gene Usage in Human T Cells Stimulated with SEE and SED
Author(s) -
LIU J. L.,
HODARA V. L.,
WIGZELL H.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03461.x
Subject(s) - sed , superantigen , t cell receptor , microbiology and biotechnology , stimulation , peripheral blood mononuclear cell , gene , cd3 , t cell , polymerase chain reaction , biology , gene duplication , immunology , cd8 , genetics , antigen , endocrinology , in vitro , immune system
Vα gene usage in human T cells stimulated with SEE and SED was investigated by using polymerase chain reaction with specific primers. Vβ8 T cells from normal blood donors PBMC were sorted at day 5 after stimulation with SEE and analysed for TCR‐Vα gene usage. Whole T cells stimulated with anti‐CD3 MoAb or SED were also analysed and compared at different time points after stimulation. There was no biased Vα gene usage found as a response to either of the two superantigens. These results show that Vα gene usage of human T cells stimulated with SEE or SED is normal.