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T Cells from the Peripheral Blood of Coeliac Disease Patients Recognize Gluten Antigens when Presented by HLA‐DR, ‐DQ, or ‐DP Molecules
Author(s) -
GJERTSEN H. A.,
SOLLID L. M.,
EK J.,
THORSBY E.,
LUNDIN K. E. A.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03414.x
Subject(s) - coeliac disease , human leukocyte antigen , peripheral blood , immunology , gluten , antigen , medicine , disease , biology , pathology
Coeliac disease (CD) is a T‐cell mediated immunological disease of the small intestine which is precipitated in susceptible individuals by ingestion of gluten. We recently reported that gliadin‐specific T cells can be found in the small intestinal mucosa of CD patients, and that a preponderance of these T cells was restricted by the CD‐associated DQ(%aL*0501, βl*0201)heterodimer. Here we report studies on whether the same is found for gliadin specific T cells in the peripheral blood of CD patients. T‐cell responses towards gluten antigens in vitro were found for both most CD patients and healthy controls. Gluten‐specific T‐cell clones (TCC) were established from four CD patients. Although a large proportion of these TCC were restricted by DQ molecules, including the CD‐associated DQ(α1*0501, β1*0201) heterodimer, several were restricted instead by DR or DP molecules. Thus, gluten‐derived peptides can be presented to T cells by several different HLA elass‐II molecules, and the preferential DQ(#aL1*0501, β1*0201) restriction of gluten‐specific T cells in the small intestinal mucosa of CD patients is less pronounced than for similar T eells in the peripheral blood.