Premium
Analysis of Oct2‐Isoform Expression in Lipopolysaccharide‐Stimulated B Lymphocytes
Author(s) -
JOHANSSON K.,
SIGVARDSSON M.,
LEANDERSON T.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03409.x
Subject(s) - gene isoform , lipopolysaccharide , microbiology and biotechnology , biology , expression (computer science) , immunology , genetics , gene , computer science , programming language
Oct2‐isoform expression in splenic B cells stimulated with lipopolysaccharide or lipopolysaccharide plus phorbol‐di‐butyrate was analysed by cDNA cloning. The frequency of Oct2‐positivc clones was 1/15, 000 in both libraries. Two new isoforms were found that generate novel amino‐ or carboxy‐terminal sequences. An isoform lacking exon 11 destroyed the carboxy‐terminal leucin‐zipper region and introduced a frame shift creating a novel, proline‐rich carboxy terminus. A new exon containing a highly basic region (4c) was characterized, between exons 4 and 5. This exon was inserted between glutamine‐rich regions 2 and 3, carboxy terminal of a tentative leucine‐zipper structure. In addition, a new combination isoform containing Oct2a's amino terminal insert (exon 7a) and Oct2b's carboxy terminal insert (exon 13) was found that created a novel large isoform, Oct2ab. More frequent use of the classical Oct2a and Oct2b isoforms was observed in the lipopolysaccharide‐stimulated B cells, while a preference for the Oct2ab and Oct2ba isoforms was observed in lipopolysaccharide plus phorbol‐di‐butyrate‐lreated cells.