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Mannan‐Binding Protein and Bovine Conglutinin Mediate Enhancement of Herpes Simplex Virus Type 2 Infection in Mice
Author(s) -
FISCHER P. B.,
ELLERMANNERIKSEN S.,
THIEL S.,
JENSENTUS J. C.,
MOGENSEN S. C.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03398.x
Subject(s) - collectin , herpes simplex virus , infectivity , biology , virology , lectin , virus , microbiology and biotechnology , antibody , vero cell , immunology , immune system , innate immune system
A broad range of plant lectins have recently been shown to inhibit the infectivity of herpes simplex virus type I (HSV‐1) in viiro . We decided to investigate the role of mammalian Icctins in infection witb herpes simplex virus. Two lectins, conglutinin and mannan‐binding protein (also called mannose‐binding protein. MBP). belonging to the collectin family of lectins, were examined. Four week‐old BALB/c mice were injected subcutaneously with 100 μg bovine conglutinin or 50 μg human MBP 1 day before intravenous infection with 5 × 10 4 PFU of herpes simplex virus type 2 (HSV‐2). A three‐fold increase in virus titre of the liver was observed on day 3 of the infection in the mice pretreated with conglutinin or MBP. whereas no effect was seen on days I and 5. In a standard plaque assay using Vero cells we were not able to demonstrate reproducibly either infection inhibition or infection enhancement, when virus was pre‐incubated with differing concentrations ofthe collectins. Tbe concentrations used were similar to tbose used by us in livo , and by others in in vitro experiments showing inhibition of the infectivity of HSV‐1 with plant lectins. In an ELISA with HSV‐2 antigens captured on anti‐HSV‐2 antibodies, calcium‐dependent and carbohydrate inhibitabte binding of the collectins was observed. Our results indicate that the effect of endogenous mammalian collectins in vivo may not be neutralization as suggested by the data using plant lectins. Instead, the previously described opsonizing activity of the mammalian collectins may provide the virions witb an alternative port of entry into cells leading to infection enhancement.