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Intra‐Embryonic Haemopoietic Cells and Early MHC Expression
Author(s) -
LAMPISUO M.,
LASSILA O.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03378.x
Subject(s) - embryonic stem cell , antigen , biology , homing (biology) , major histocompatibility complex , microbiology and biotechnology , stem cell , mhc class i , immunology , endothelial stem cell , chimera (genetics) , genetics , in vitro , gene , ecology
In the avian embryo the haemopoietic stem cells originate from the intra‐embryonic area near dorsal aorta. The surface‐marker expression of haemopoietic stern cells and their potential to produce different haemopoietic cells are still largely unknown. The surface antigen expression and particularly the MHC antigen expression on intra‐embryonic haemopoietic cells was studied. Expression of B‐F antigens, homologous to mammalian MHC class‐I antigens, was found already on embryonic day (ED) 5. The first B‐L antigens, analogous to mammalian MHC class‐II antigens, were detected also from ED5 onwards. The appearance of surface antigens defined by MoAbs T10A6 and 3–298 during embryogenesis also was studied. The antigen defined with T10A6 was detected from ED4 onwards on endothelial cells but not on haemopoietic cells in the para‐aortic region. The first 3–298 + haemopoietic cells were found on ED6, whereas endothelial cells were negative. These findings imply that some surface markers are shared with haemopoietic and endothelial cells indicating either a common embryonic origin or the importance of these molecules in embryonic stem‐cell homing.