Premium
Differential Effects of IL‐10 on Proliferation and Cytokine Production of Human γ/δ and α/β T cells
Author(s) -
SCHLAAK J. F.,
HERMANN E.,
GALLATT H.,
BOSCHENFELDE KH. MEYER ZUM,
FLEISCHER B.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03362.x
Subject(s) - cytokine , t cell , biology , t cell receptor , peripheral blood mononuclear cell , immunology , cell growth , immune system , gamma delta t cell , microbiology and biotechnology , in vitro , biochemistry
Gamma/delta TCR bearing T lymphocytes represent a T‐cell subset whose functional relevance remains unclear. Nevertheless these T cells may play a role in the early immune reponse against bacteria. Until now the regulatory mechanisms on this response have not been investigated. The study described here evaluated the immunoregulatory effects of Interleukin‐10 on γ/δ and α/β TCR‐positive T‐cell clones and freshly isolated peripheral‐blood mononuclear cells (PBMC). IL‐10 has been shown previously to inhibit lectin and antigen‐induced proliferation and cytokine production by α/β T cells. The results outlined below show that rhIL‐10 strongly inhibits lectin‐induced production of IFN‐γ, TNF‐α. IL‐2, and to a lesser degree proliferation and IL‐4 production of both T‐cell subsets. As IL‐10 did not inhibit proliferation but at the same time strongly suppressed cytokine production in various experiments, the hypothesis that it could function as a growth factor for human T cells as has been described for murine thymoeytes was tested. The data demonstrate that, although the γ/δ T‐cell clones tested do not produce IL‐10 they can use it as a growth factor in combination with IL‐2, IL‐4 or alone. Furthermore, IL‐10 has the same properties on human α/β T‐cell clones and PBMC. In summary, it is shown that IL‐10 has pleiotropic effects on γ/δ and α/β TCR + T cells by inhibiting lectin‐induced cytokine production and by acting as a growth factor for these cells alone or in combination with IL‐2 or IL‐4.