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Ligation of Leukocyte Function‐Associated (LFA) Molecule‐1 Provides an Accessory Signal for T‐Cell Activation with Pokeweed Mitogen
Author(s) -
WALLAYS G.,
CEUPPENS J. L.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03352.x
Subject(s) - pokeweed mitogen , t cell , lymphocyte function associated antigen 1 , monocyte , antigen presenting cell , cell adhesion molecule , microbiology and biotechnology , biology , immunology , immune system , intercellular adhesion molecule 1 , in vitro , concanavalin a , biochemistry
The ligation between Leucocyte‐Function‐Associated Molecule I (LFA‐1) and Intercellular Adhesion Molecules (ICAM) is thought to be an important component in the stimulatory interaction between antigen‐presenting cells (APC) and T cells. Similar to antigenic stimulation, T‐cell stimulation with pokeweed mitogen (PWM) is highly dependent on monocytes as accessory cells. This is partly a consequence of the requirement for mitogen presentation by the monocytes. The study described here addressed the question of whether LFA‐1 ligation by accessory cells influences the activation of T cells with PWM. To avoid multiple costimulatory interactions between T cells and monocytes. experiments were performed with purified T cells, which were stimulated with PWM bound on autologous red blood cells (PWM‐RBC). Binding on the RBC substituted partly for PWM presentation by the monocytes. Anti‐LFA‐1 MoAbs were presented in the immobilized form in order to mimick LFA‐1 ligation by cell‐bound ICAM. Three out of three different MoAbs against the β‐chain of the LFA‐1 molecule (CD18) and two out of three MoAbs against the α‐chain (CD11a) had an enhancing effect on T‐cell proliferation, Proliferation was increased further by simultaneous addition of interleukin (IL)‐1 β and IL‐6. Ligation of the LFA‐1 molecule was found to enhance IL‐2 production and tumour necrosis faclor‐α (TNF‐α) production. The results suggest that interaction of LFA‐1 with ICAM on the monocytes contributes to the accessory signal activity of monocytes in T‐cell activation with PWM.