Premium
Major Histocompatibility Complex Class‐II Alleles in Primary Biliary Cirrhosis
Author(s) -
ZHANG L.,
WEETMAN A. P.,
BASSENDINE M.,
OLIVEIRA D. B. G.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03346.x
Subject(s) - primary biliary cirrhosis , allele , major histocompatibility complex , biology , restriction fragment length polymorphism , polymerase chain reaction , typing , immunology , histocompatibility , genetics , allele frequency , microbiology and biotechnology , human leukocyte antigen , antigen , gene
The major histocompatibility complex (MHC) class‐II alleles at the DRBI, DQBl and DPBl loci were investigated in 40 patients with primary biliary cirrhosis (PBC) and 43 local healthy controls. Restriction fragment length polymorphism (RFLP) was used for DRBI typing. DQBl and DPBl regions were amplified using the polymerase chain reaction (PCR) and then probed with 32 P‐labelled allele‐specific oligonucleotide probes. There was an increased frequency of DR8 (10% compared to 4% in controls), and a decreased frequency of DR2 (18% compared with 28% in controls) in patients with PBC, but the differences were not significant. There were no significant differences for the other DR alleles, the seven DQ alleles, or the eight DP alleles. In conclusion, no significant MHC class‐II associations with primary biliary cirrhosis have been demonstrated.