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Schistosoma mansoni Egg‐Primed ThO and Th2 Cells: Failure to Down‐regulate IFN‐γ Production Following In Vitro Culture
Author(s) -
VELLA A. T.,
PEARCE E. J.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03333.x
Subject(s) - schistosoma mansoni , in vitro , biology , microbiology and biotechnology , cell culture , interferon gamma , andrology , immunology , chemistry , schistosomiasis , biochemistry , helminths , medicine , genetics
Schistosoma mansoni eggs induce a rapid and pronounced T h response which, based on cytokine secretion patterns, at day 3 post priming is T h 0)‐like and at day 10 is T h 2‐like. To establish whether or not the day‐3 cells have been programmed in vivo to develop into T h 2 cells, they were cultured for 7 days to become in vitro equivalents of day‐10 in vivo cells. Following this culture period, the population was approximately 75% CD4 + , 22% CD8 + , 6% B220 + and capable of producing IL‐2, IFN‐γ, IL‐4, ‐5 and ‐10 upon stimulation. This T h 0‐like status was confirmed by the observations that in response to mitogen IL‐4 and IFN‐γ production are both CD4 + ‐cell dependent and that IFN‐γ and IL‐4 are produced concomitantly by single cells. These data suggest that T h O cells persist in vivo , but are incapable of secreting IFN‐γ at day 10 due loan inhibitory factor which does not develop or is labile in vitro . This concept is supported by ihc surprising observation that day‐10 LN cells, which are T h 2‐like immediately ex‐vivo , rapidly gain the ability to secrete IFN‐γ following a short period of culture.