Premium
Activated Natural Killer Cells Suppress Myelopoiesis in Mice with Severe Combined Immunodeficiency
Author(s) -
PISA P.,
SITNICKA E.,
HANSSON M.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb03330.x
Subject(s) - myelopoiesis , lymphokine activated killer cell , natural killer cell , splenocyte , lymphokine , progenitor cell , biology , immunology , bone marrow , k562 cells , myeloid , spleen , cytotoxicity , stem cell , t cell , interleukin 21 , microbiology and biotechnology , immune system , in vitro , leukemia , biochemistry
The in vivo effect of natural killer (NK) cell activation on aulologous myelopoiesis was studied in an environment deficient of functional Tand B cells. Administration of 3.6‐bis[2‐(Dimethylamino)‐ethoxy]‐9H‐xanthen‐9‐one dihydrochloride) Tilorone) or recombinant interleukin‐2 (rIL‐2) to mice with severe combined immunodeficiency (C. B. ‐I7 scid/scid) resulted in an increase in YAC‐1 lysis by their splenocytes as well as bone marrow cells. Recombinant IL‐2 furthermore led to a fivefold increase in the cellularity of the spleen. When assayed against human NK/lymphokine‐activated killer (LAK) target, K562 cell line. the IL‐2‐activated mouse cells exhibited no cytotoxicity across the species barrier. Both agents induced a profound suppression of myelopoietic progenitor cells as measured in a 7‐day granulocyte‐macrophage colony forming cell (GM‐CFC) assay. We conclude that the presence of neither functional T nor B cells is necessary for NK cells to mediate inhibition cf myelopoiesis in the autologous host.