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Modification of Lymphocyte and Monocyte Functional Activity by Polymorphonuclear Neutrophils in HIV Infection
Author(s) -
GABRILOVICH D. I.,
SHEPELEVA G. K.,
SEREBROVSKAYA L. V.,
AVDEEVA L. A.,
POKROVSKY V. V.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb03319.x
Subject(s) - monocyte , polymorphonuclear leukocyte , immunology , human immunodeficiency virus (hiv) , lymphocyte , granulocyte , medicine , biology , in vitro , biochemistry
The aim of this study was to determine whether potymorphonuclear neutrophils (PMN) can modify the immune response in HIV cases. Supernatants of PMN (PMNS) from 33 HIV‐infected patients (16 with lymphoadenopathy syndrome, 17 with AIDS‐related complex) were tested for their influence on the functional activity oflymphocytes and monocytes from 6 healthy donors. PMNS from another 6 healthy donors comprised a control group. It was found that PMNS from HIV‐infected patients, but not from healthy donors, induced suppression oflymphocyte proliferative response and down‐regulation of CD8 receptor expression on lymphocytes. Decrease of NK‐cell cytotoxicity in the presence of PMNS from HIV‐infecled patients was the same as that from healthy donors. PMNS did not influence the production of anti‐HIV antibody by lymphocytes from HIV‐infected patients, as well as non‐specific IgG by lymphocytes from healthy donors. PMNS effect on functional activity of lymphocytes was blocked completely after treatment of PMN by catalase and superoxidedismutase. At the same time PMNS from HIV‐infected patients but not from healthy donors induced increased production of TNF‐a by monocytes and up‐regulation of monoeyte phagoeytosis. These effeets were independent of catalase and superoxide dismutase and were not abrogated by antibody against IL‐1, IL‐8, TNF‐γ, IFN‐γ or IFN‐γ.