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Partial Biochemical Characterization and Purification of IgG2b Inducing Factor as a New Cytokine from Synovial Fluid of Patients with Rheumatoid Arthritis
Author(s) -
ABEDIVALUGERDI M.,
RIDDERSTAD A.,
STRÖM H.,
MÖLLER G.,
MOLLER E.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb03314.x
Subject(s) - synovial fluid , rheumatoid arthritis , immune system , antibody , cytokine , in vivo , lipopolysaccharide , in vitro , chemistry , immunology , rheumatoid factor , arthritis , biology , medicine , biochemistry , pathology , osteoarthritis , alternative medicine , microbiology and biotechnology
Rheumatoid arthritis synovial fluid (RA‐SF) contains a novel biological activity, which selectively induces IgG2b antibody production in lipopolysaccharide (LPS)‐activated mouse spleen cells in vitro and in vivo . Our previous studies have shown that this activity is not functionally identical to otherwell‐known cytokines and interleukins. In this study we demonstrate the partial purification and biochemical characterization of the IgG2b inducing activity in RA‐SF. Biochemical characterization revealed that the IgG2b inducing activity in RA‐SF has the following properties: it is a protein, sensitive to pH > 11 and < 4, which is precipitated by 50% of saturated ammonium sulphate and has a molecular weight of 50–70 kDa; it binds to Cibacron‐blue and heparin and its activity is not mediated by immunoglobulins or immune complexes, which are present in RA‐SF. Biochemical characteristics of the IgG2b inducing activity also differ from other cytokines and interleukins. The term IgG2b inducing factor is proposed for this novel activity.