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Gluten Stimulation of Coeliac Mucosa In Vitro Induces Activation (CD25) of Lamina Propria CD4 H T cells and Macrophages but no Crypt‐Cell Hyperplasia
Author(s) -
HALSTENSEN T. S.,
SCOTT H.,
FAUSA O.,
BRANDTZAEG P.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb03245.x
Subject(s) - il 2 receptor , microbiology and biotechnology , lamina propria , biology , intraepithelial lymphocyte , t cell , population , cd8 , antigen , pathology , immunology , medicine , immune system , epithelium , environmental health
Jejunal biopsy specimens from 10 patients with treated coeliac disease and seven non‐coeliac controls were challenged in vitro with peptic‐tryptic gluten digest. Mucosal T cells were examined in situ by three‐colour immunofluorescence staining for expression of the activation marker CD25 (the p55 α‐chain of intcrlcukin‐2 receptor) and the nuclear proliferation marker revealed by monoclonal antibody Ki‐67. Intraepithelial T cells expressed CD25 rarely whereas the proportion of activated lamina propria T cells increased ( P < 0.002) from median 2.8% (cultured with 20% fetal calf serum alone for 24–48 h) to 10.0% after 24h with gluten ( n= 10; range 1.1–17.4%) and to 10.4% after 48 h (n = 7; range 1.4–17.5%). Such gluten‐induced increase of CD25 + T cells was not observed in specimens from non‐coeliac control subjects. Crypt‐cell hyperplasia and T‐cell proliferation (Ki‐67 + ) were observed neither in the coeliac nor in the control mucosae after gluten stimulation. Three‐colour staining combining a polyclonal antibody reagent to CD3 and a monoclonal antibody to CD25 with a monoclonal antibody to CD45RO, CD4. CDS, the p75 β‐chain of intcrleukin‐2 receptor, integrin χ E β7, or HLA‐DR showed that most of the CD25 + T cells (> 90%) were CD4 + CDS, co‐expressed CD45RO and the p75 β‐chain. and often also the integrin χ E β7 but not HLA–DR. In addition to these activated T cells, a dominating population of CD25 + CD3 ‐ CD4 + subepithelial pan‐HLA–class I + macrophages (CD68 + ) with variable expression of the p75 β‐chain was often induced by gluten challenge.