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B Cells Carrying Surrogate Receptors in their Membranes Process and Present Antigen to Specific Murine T Cells
Author(s) -
GONTIJO C. M.,
MÖLLER G.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb03242.x
Subject(s) - ovalbumin , antigen , cd40 , priming (agriculture) , antibody , antigen presenting cell , microbiology and biotechnology , cytotoxic t cell , chemistry , t cell , biology , immunology , immune system , in vitro , biochemistry , botany , germination
A palmitate‐conjugate derivative of ovalbumin which can be inserted into the membrane of B cells has been prepared. The ability of these cells to act as antigen‐presenting cells for specific T lymphocytes obtained from immunized mice was tested. It was found that the conjugates were more efficiently processed and presented than the naive form of the antigen. Palmitate‐conjugated antibodies specific to ovalbumin were also inserted into the cell membrane of normal B lymphocytes. These cells were pulsed with the antigen and tested as antigen‐presenting cells for T cells obtained from immunized mice. The antibody‐decorated B cells presented ovalbumin more efficiently than non‐decorated controls. Whether antibody‐decorated, antigen‐pulsed B cells could prime T cells in viro was investigated. Some priming activity was found.