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Phenotypic Characterization of Splenic T Cells from Mice Infected with Plasmodium chabaudi chabaudi
Author(s) -
LANGHORNE J.,
PELLS S.,
EICHMANN K.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb03235.x
Subject(s) - plasmodium chabaudi , phenotype , biology , spleen , virology , immunology , genetics , malaria , gene , plasmodium falciparum , parasitemia
T cells from spleens of mice infected with the erythrocytic stages of Plasmodium chabaudi chabaudi have been analysed with respect to their expression of surface molecules CD3, CD4 and CDS and T‐cell receptor (TCR)αβ and γδ. The majority of T cells from infected mice were αβTCR + . However, there was an increase of approximately 8–10‐fold in the proportion and total number of γδ T cells. Immunocytochemical analysis of sections of spleens taken from infected C57BL/6 mice during a primary infection showed that this increase took place particularly in the non‐lymphoid areas. Within the αβ TCR + T‐cell population, both CD4 + T cells and CD8 + T cells were represented in proportions similar to those observed in normal uninfected mice. Stimulation of splenic T cells from infected mice with P. chabaudi‐ infected erythrocytes in vitro resulted ina blasted cell population composed predominantly of αβTTCR + T cells with no preferential expansion of γβTCR + T cells. There was no evidence of superantigen‐like stimulation of T cells bearing particular Vβ chains of the TCR. The representation of the different Vβ chains within the population was not significantly different from that seen in uninfected mice.