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Do Human Peyer's Patches Contribute to the Intestinal Intraepithelial γ/δ T‐Cell Population?
Author(s) -
FARSTAD I. N.,
HALSTENSEN T. S.,
FAUSA O.,
BRANDTZAEG P.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb02587.x
Subject(s) - lamina propria , intraepithelial lymphocyte , biology , microfold cell , microbiology and biotechnology , population , cd8 , immunofluorescence , pathology , antigen , epithelium , immunology , antibody , immune system , medicine , genetics , environmental health
T‐cell receptor γ/δ† (TcRγ/γ†) lymphocytes in human Peyer's patches (PP) adjoining ileal mucosa were studied by monoclonal antibodies with paired immunofluorescence staining in situ and by flowcytomelric phenotyping of isolated cells. The proportion of γδ† T cells in the follicle‐associated epithelium outside the M‐cell areas (median 4·I%, range 2·2–30·1%,) was similar to that in mucosal villous epithelium (median 4·4%, range 0·5 30·5%). Most intraepithelial °/δ cells (∼90%) expressed CD45RO but only a few expressed CD8 (<10%) and none l‐selectin; a dominating subset (median 46%) employed the Vδl/JδI gene product (range 22–100%). The M‐cell areas lacked γ/δ cells but contained clustered CD20 + andCD3+ lymphocytes. Thesubepithelial PP dome area and interfollicular(T‐cell) zones, as well as the mucosal lamina propria. contained very few γ/δ cells (median 1·7%, range 0·4–8·9%) which were dominated (88–100%) by the Vγ 2‐encoded subset. Those in the dome area and lamina propria were often (∼75%) CD45RO+ (range 44·90%) while very few (∼2%) expressed i.‐selectin (range 0·15%). By contrast, CD45RO expression on γ/δ cells in the PPT‐cell zones was relatively low (∼46%) and that of l‐selectin relatively high (∼43%). In conclusion, TcR γ/δ + cells are quite rare in human PP and belong mainly to the Vδ 2 ‐encoded subset, thus being different from most inlraepithelial γδ cells that probably have another origin. The L‐selectin ‘fraction of PPγ/δ cells presumably represent newly recruited ‘naïve’ T lymphocytes while CD45RO + γ/δ cells both in PP and lamina propria arc probably antigen‐primed.