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Organ‐Specific Regulation of Interferon‐γ, Interleukin‐2 and Interleukin‐2 Receptor during Murine Infection with Trypanosoma cruzi
Author(s) -
ROTTENBERG M. E.,
SUNNEMARK D.,
LEANDERSSON T.,
ÖRN A.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb02572.x
Subject(s) - spleen , lymph node , biology , interferon gamma , interleukin 2 , cytokine , lymph , immunology , trypanosoma cruzi , messenger rna , microbiology and biotechnology , receptor , interferon , medicine , pathology , parasite hosting , gene , biochemistry , world wide web , computer science
We have studied the expression of IFN‐γ, IL‐2 and IL‐2 receptor (IL‐2R) at the mRNA and protein levels in spleen and lymph node cells from Trypanosoma crizi ‐infected BALB/c mice. At 21 days post infection (dpi) (peak of parasitaemia), spleen cells stimulated with Con A for 16 h showed a reduced IFN‐γ, IL‐2 and IL‐2R mRNA production compared with non‐infected controls. Lymph node cells obtained at 4, 21 or 60 dpi produced similar amounts of IFN‐γ, IL‐2 or IL‐2R transcripts after mitogen stimulation as uninfected controls. Spleen cells obtained at 21 dpi showed a lower Con A proliferative response and IL‐2R expression compared with non‐infected controls, while the proliferation and IL‐2R expression of lymph node cells at 21 dpi was unaltered. Supernatantsfrom 48 h Con A‐stimulated spleen and lymph node cells from mice at 21 dpi had very low levels of IL‐2 but contained significantly higher levels of IFN‐γ compared with the supernatants of cells from non‐infected mice. The latter phenomenon correlates with an accelerated rate of IFN‐γ mRNA accumulation.