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Evidence for an Ongoing Role of Class I Histocompatibility Molecules for the Production of Interleukin‐2 in Response to Suboptimal Concentrations of Phytohaemagglutinin
Author(s) -
HASHIMOTO S.,
McCOMBS C. C.,
MICHALSKI J. P.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb02570.x
Subject(s) - phytohaemagglutinin , major histocompatibility complex , interleukin 2 , antigen , biology , antibody , receptor , microbiology and biotechnology , immunology , cytokine , immune system , biochemistry
To examine the role of cellular interactions involving class I histocompatibility antigens in the response to low concentrations of phytohaemagglutinin, we studied the effect of antibodies to components of these antigens on proliferative responses, interleukin‐l and interleukin‐2 production, and IL‐2 receptor expression. Antibody to human β 2 ‐microglobulin (β 2 m) had an inhibitory elTect both on IL‐2 accumulation at 48 h of culture and on the proliferative response 24 h later. Exogenous IL‐2 completely reconstituted the inhibited proliferative responses, and also restored the modest decrease in IL‐2 receptor expression that was induced by anli‐βm. Pretreatment of either purified monocytes or T cells with anti‐β 2 m had a simiiar inhibitory effect both on proliferation and on interleukin‐2 production. By contrast. IL‐1 production by LPS‐ or silica‐stimulated monocytes was not affected by this antibody. Kinetic experiments demonstrated that anti‐β 2 m was equally inhibitory when added at the initiation of culture or after 24 h. and significant inhibition occurred when the antibody was added as late as 48 h. Our results are consistent with an ongoing role for class I antigens in the cellular interactions between lymphocytes and accessory cells required for the production of IL‐2.