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Adhesion Molecules Involved in Protein Kinase A‐ and C‐Dependent Lymphocyte Adherence to Microvascular Endothelial Cells
Author(s) -
TURUNEN J. P.,
USTINOV J.,
RENKONEN R.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb02555.x
Subject(s) - adhesion , cell adhesion molecule , lymphocyte , microbiology and biotechnology , chemistry , protein kinase a , cell adhesion , protein kinase c , immunology , medicine , biology , kinase , organic chemistry
A twofold increase in lymphocyte adherence to rat microvascular endothelial cells (EC) was achieved by incubating EC for 4 h with IL‐1α or dibulyryl‐cAMP (stimulators of protein kinase A, PKA) and PMA (stimulator of protein kinase C, PKC). Monoclonal antibodies anti‐CD11a, anti‐CD18 (LFA‐I) and anti‐CD49d (VLA‐4α) significantly inhibited the increased lymphocyte binding to IL‐lα‐induced EC, anti‐CD 18 and to a lesser extent anti‐CD11a and anti‐CD49d to dibutyryl‐cAMP‐induced EC, whereas only anti‐CD11a and anti‐CD18 monoclonal antibodies inhibited PMA‐induced lymphocyte binding. These findings suggest that stimulation of PK A and PKC induces lymphocyte binding to EC via different adhesion molecules.