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Up‐Regulation by Cyclosporine (CsA) of the In Vitro Release of Soluble CD23 (sCD23) and of the In Vitro Production of IL‐6 and IgM
Author(s) -
HORNUNG N.,
DEGIANNIS D.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb01727.x
Subject(s) - in vitro , cd23 , in vivo , cytokine , immune system , biology , immunology , microbiology and biotechnology , chemistry , immunoglobulin e , antibody , biochemistry
In the present study, we examined the effect of CsA on the in vitro production of Ig and on the in vitro production of molecules known to have B‐cell growth and differentiation activities, such as IL‐6 and sCD23. For the purpose of this study, we developed an experimental In vitro system closely resembling an In vivo model of ongoing B‐cell activation. Pre‐activated B cells proliferated and produced IgM optimally when they were re‐cultured in the presence of IL‐2/IL‐6. CsA down‐regulated the IL‐2/IL‐6‐induced proliferative responses of pre activated B cells by at least 50%., but it up‐regulated IgM production in the same experiments. This up regulating effect was not cytokine‐related since it was also seen when cells were re‐cultured in the absence of any cytokines. Optimal release of sCD23 was observed when SAC‐pre‐activated B cells were re cultured in the presence of IL‐4 or IL‐4 plus IL‐2 and CsA up‐regulated significantly the release of this molecule in these cultures. Finally, CsA was shown to inhibit PHA‐induced cell proliferation of PBMC and to up‐rcgulate IL‐6 production in the same cultures. We conclude that CsA can amplify in vitro both the production of Ig and the release of sCD23 by pre activated B cells. This finding, in combination with the CsA‐induced up‐regulation of lectin‐induced IL‐6 production, may have clinical implications in disease states with an ongoing immune activation, where prolonged administration of CsA might be anticipated.