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Phenotypic and Molecular Characterization of Human Monoclonal TCR γ/δ T‐Cell Lines from Jejunum and Colon of Healthy Individuals
Author(s) -
ROBIJN R. J.,
BLOEMENDAL H.,
JAINANDUNSING S.,
WIEGMAN L. J. J. M.,
VANBERGEHENEGOUWEN G. P.,
LOGTENBERG T.,
KONINGSBERGER J. C.
Publication year - 1993
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1993.tb01721.x
Subject(s) - t cell receptor , biology , monoclonal antibody , delta , microbiology and biotechnology , population , phenotype , intraepithelial lymphocyte , genetics , t cell , immunology , gene , antibody , medicine , epithelium , immune system , environmental health , engineering , aerospace engineering
We have perfortned a phenotypic and molecular analysis of monoclonal TCRγ/δ T‐cell lines derived from jejunal and colonic biopsies of healthy individuals. Flow cytometric analysis employing a panel of 24 monoclonal antibodies (MoAbs) demonstrated that intestinal TCRγ/δ intraepithelial lymphocytes (IEL) constitute a phenotypically heterogeneous population. Nucleotide sequence analysis of expressed TCRδ variable (V) regions revealed the dominant utilization of the Vδ2 and Dδ3 gene segments and frequent rearrangement of Jδ3. IEL Vδ regions displayed extensive junctional diversity as a result of N and P insertion and the utilization of Dδ3 in all three reading frames. The results demonstrate that intestinal TCRγ/δ T cells from healthy individuals constitute a phenotypically heterogeneous population expressing Vδ regions that differ from their systemic counterparts.